Journal: Oxidative Medicine and Cellular Longevity

Article Title: Inhibiting Cardiac Mitochondrial Fatty Acid Oxidation Attenuates Myocardial Injury in a Rat Model of Cardiac Arrest

doi: 10.1155/2021/6622232

Figure Lengend Snippet: Myocardial injury at 1, 3, and 6 hours following return of spontaneous circulation (ROSC) in the CPR and CPR + Trimetazidine (TMZ) groups compared with the Sham group: (a) cardiac troponin I (CTNI); (b) mean aortic pressure (MAP); (c) left ventricular ejection fraction (EF); (d) representative echocardiograms in three groups at 6 hours following ROSC; (e) pathological examination of myocardial tissue in three groups at 6 hours following ROSC. Magnification ×200. β p < 0.05, ∗ p < 0.01 versus Sham group; # p < 0.05, $ p < 0.01 versus CPR group.

Article Snippet: Cardiac troponin I (CTNI) levels in serum were detected using a Rat CTNI ELISA kit (CUSABIO, China).

Techniques:

Journal: Pharmaceutics

Article Title: Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by Erythropoietin

doi: 10.3390/pharmaceutics15041049

Figure Lengend Snippet: Production of angiogenic cytokines in mMSCs. ( a ) Representative microscopic images of the mMSCs. Left phase contrast image. Original magnification ×40. Right, red fluorescence indicates PDGFR-α. PDGFR-α was ubiquitously expressed in the mMSCs. Nuclei were stained with DAPI (blue). Bar = 50 µm. ( b ) Concentration levels of VEGF and HGF in the conditioned medium. N = 6. * p < 0.05 vs. BMMSCs. # p < 0.01 vs. skMCs. mMSCs, mesenchymal stem/stromal cells derived from skeletal muscle; PDGFR-α, platelet-derived growth factor receptor-α; DAPI, 4’,6-Diamidino-2-phenylindole; VEGF, vascular endothelial growth factor; HGF, hepatocyte growth factor; BMMSCs, bone marrow mesenchymal stem/stromal cells; skMCs, skeletal muscle cells.

Article Snippet: Rat skeletal muscle cells (skMCs) and human umbilical cord vein endothelial cells (HUVECs) were purchased from Cell Applications Inc. (San Diego, CA, USA).

Techniques: Fluorescence, Staining, Concentration Assay, Derivative Assay

Journal: Pharmaceutics

Article Title: Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by Erythropoietin

doi: 10.3390/pharmaceutics15041049

Figure Lengend Snippet: Proliferation of cultured mMSCs by Erythropoietin (Epo). ( a ) Left, Epo-R (green) expression in mMSCs. Nuclei were stained with DAPI (blue). NC, negative control. Bar = 50 µm. Right, western blot analysis. Epo stimulated the phosphorylation of Akt and STAT3 in the mMSCs. Epo-R, erythropoietin receptor. ( b ) Efficacy of Epo stimulation on the propagation of HUVECs, skMCs, and mMSCs. N = 3 to 6. # p < 0.01. HUVECs, human umbilical cord vein endothelial cells.

Article Snippet: Rat skeletal muscle cells (skMCs) and human umbilical cord vein endothelial cells (HUVECs) were purchased from Cell Applications Inc. (San Diego, CA, USA).

Techniques: Cell Culture, Expressing, Staining, Negative Control, Western Blot, Phospho-proteomics

Journal: Biochemistry and Biophysics Reports

Article Title: Actin exposure upon tissue injury is a targetable wound site-specific protein marker

doi: 10.1016/j.bbrep.2016.05.013

Figure Lengend Snippet: Actin recognition in injured sites of various tissues. (A). Traumatized rat spleen (left), kidney (middle) or muscle (right) tissues were treated with fluoresceinated (FITC-labeled) antibody against actin (top panels) or IgG as negative control (bottom panels). (B). Quantitative analysis (mean and standard deviation) of image intensities at the wounded surface versus the neighboring healthy areas for spleen, kidney and muscle tissues treated with anti-actin (top panel) or IgG (bottom panel) antibodies.

Article Snippet: Rat skeletal muscle tissue was purchased from BioreclamationIVT (Liverpool, NY).

Techniques: Labeling, Negative Control, Standard Deviation